Planned Papers
Title: "Sensitive aflatoxin B1 determination using magnetic particles-based enzyme-linked immunosorbent assay"
Author: Madalina Tudorache 1 and Camelia Bala 1,2 *
1 Laboratory of Quality Control and Process Monitoring, 2
Department of Analytical Chemistry, University of Bucharest, 4-12
Regina Elisabeta Blvd., 030018 Bucharest, Romania; * e-mail:
camelia.bala@g.unibuc.ro
Abstract: New
alternative analysis for Aflatoxin B1 determination had been developed
as magnetic particle-based enzyme-liked immunosorbent assay (mp-ELISA).
The method principle is based on conventional competitive ELISA where
the anti-Aflatoxin B1 antibody is immobilized on the magnetic particles
surface. A permanent magnet placed close to the microplate walls allows
manipulating easily the magnetic particles together with the
immobilized reagent (e.g. anti-Aflatoxin B1 antibody). As a
consequence, the antibody is conveniently distributed into the volume
of the microtitre plate well improving the immuno-interaction
efficiency. Therefore, the equilibrium of the immuno-interaction will
be achieved faster than in classical ELISA (10 min for mp-ELISA),
decreasing dramatically the analysis time (maximum 20 min). The
mp-ELISA involves fewer steps, larger interface contact liquid-solid,
more stable and efficiently bioactive surface and lesser
immuno-reagents utilised than conventional ELISA.
Different types of
anti-aflatoxin B1 antibody were tested (e.g. Ab I – polyclonal
anti-Aflatoxin B1 antibody, Ab II – polyclonal anti-Aflatoxin B1
affinity purified antibody and Ab III – monoclonal anti-Aflatoxin B1
antibody) for the mp-ELISA configuration. Also, different
immobilization procedures of the anti-Aflatoxin B1 antibody on the
magnetic particles surface were used for each type of antibody. Thus,
the antibody was covalent immobilised on the particles or affinity
immobilised after covering the magnetic particles with protein G/A. Ab
III gave the best results showing higher affinity for Aflatoxin B1 than
the others. For this case, direct immobilization of antibody on the
magnetic particles allowed to get more sensitive analysis than the
affinity immobilization procedure (LOD = 0.001 ppb for covalent
immobilization and LOD = 0.1 and 0.05 ppb for affinity immobilization
via protein G and A, respectively).
Finally, sensitive immunoassay method (mp-ELISA) was set up for Aflatoxin B1 determination at ppt level.
References
Ibraimi F, Kriz D, Lu M, Hansson L-O, Kriz K (2006) Anal. Bioanal. Chem. 384: 651-657.
Liu GD, Timchalk C, Lin YH (2006) Electroanalysis 18: 1605-1613.
Wellman AD, Sepaniak MJ (2006) Anal. Chem. 78: 4450-4456.
Wang S-F, Tan Y-M (2007) Anal. Bioanal. Chem. 387: 703-708.
Zhao X, Shippy SA (2004) Anal. Chem. 76: 1871-1877.
Sole S, Merkoci A, Alegret S (2001) TrAC 20: 102-110.
Rad AY, Yavuz H, Kocakulak M, Denizli A (2003) Macromol. Biosci. 3: 471-476.
Topic: "Quantum Dot Biosensors"
Author: Jay Nadeau, Canada Research Chair in Nanocellular Neuroscience, McGill University, Canada, Tier 2 - June 1, 2004; Tel. 514-398-8372;
E-mail: jay.nadeau@mcgill.ca
Abstract:
to be added soon
Summary
Coupling of biomolecules or complex biological
systems with electronic or optoelectronic devices is the general
principle of various biosensors. The effective performance of
biosensors requires transduction of the chemical signals generated by
the biological components to electronic signals. New methods and new
materials (functionalized nanoparticles, carbon nanotubes, etc.)
developed due to the tremendous recent success in nanotechnology pave
the way for the novel possibilities to couple biomaterials and
electronic transducers. The great importance of cooperative efforts in
bioelectronics and nanotechnology, resulting in the formulation of the
novel scientific direction named “bionanotechnology” cannot be
overestimated. The recently born scientific direction has already
attracted major interests of researches. Dimensional similarity of
biomolecules and nano-objects allow their functional coupling, thus
providing effective chemical/electronic signal transduction in the
hybrid systems. The bionano-hybrid systems keep great promise for the
development of novel biosensors, biofuel cells and biocomputing
elements. Therefore, cooperative efforts of chemists, physicists and
engineers from both science/technology areas (bioelectronics and
nanotechnology) are needed to bring these promises into reality. These
efforts should be directed to the basic science and to the
technological development solving practical issues. The present special
issue will outline the state-of-the-art in the area of
nanotechnological advances in biosensors.
Keywords
Bionanotechnology, Nanotechnology, Biosensors, Nanomaterials,
Nanostructure, NEMS, Functionalized Nanoparticles, Carbon Nanotubes,
Biofuel Cells, Biocomputing
Submission
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http://www.mdpi.org/sensors/) is a highly
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MDPI - Matthias Burkhalter - 25 September 2008